مطالعه اثر آنتی اکسیدانی عصاره تام و فلاونوییدی گیاه سس (Cuscuta lehmanniana Bunge) بر سلول های 12 PC مدل دیابتیک

Background: Mellitus (DM). The exact mechanisms of diabetic neurotoxicity are still unknown. Recently oxidativestress is introduced as one of the factors for diabetics’ neuropathies. Antioxidants also used astherapeutic agents to reduce the side effects of diabetes. In the present research the antioxidant effectsof total and flavonoid extracts of Cusseta lehmaniana Bunge on high glucose inducted Pc12 werestudied.Central and peripheral neuropathies are the most common side effects of DiabetesMethods: lehmaniana Bungs were preparedexposed to extracts. Antioxidant activity of extracts, cell viability and apoptosis were studied byDPPH, MTT, Annexin staining and Western Blotting respectively.PC12 cell line treated with high glucose was used. Total and flavonoid extract of C.. PC12 cells treated with 6X fold high glucose concentrationResults: Bungs extract increased cell viability after 3 hours pretreatment. DPPH assay demonstrated 50 μg/mlflavonoid extract can increased radical scavenging inhibits compare with ascorbic acid. Several assayswere used for evaluated of anti apoptotic effect such as: Annexin, SubG1and Western blotting forexpression of Bax protein.The MTT result showed 50μg/ml of flavonoid extract and 100μg/ml of total C. lehmanianaConclusion: lehmaniana Bungs have the potential to protect PC12 cells against glucose neurotoxicity by reducingapoptosis via increased Bax expression protein.Based on our findings, it is concluded that both total and flavonoid extract of C.